zaocys dhumnades benefits

Scientific Name: Synonymns and other Related Names: No related names recorded. arthralgia, convulsion, scabies and malignant scabiesbecause it travels 8600 Rockville Pike the bile from most other snakes show only 8 of those bands, indicating unique However, most of those methods are time and labor intensive because of the need for multiple separations, washes or repeated temperature changes. The soluble total proteins of Zaocys dhumnades produce therapeutic effect on RA possibly by inhibiting IL-1beta and TNF-alpha and promoting IL-10 expressions in the FLS. Four new snakes and a new lizard from South China. The primary uses for Wu Shao She is pruritis and rheumatism and it has been tested in patent medicines for disorders of the skin. Results: Parvathi, S. & Brindha, R. Ethnobotanical medicines of Anaimalai union. amplification with specific primer, both the genuine and 14 The results 76, 19631974 (2010). The protein and mRNA levels of IL-1beta and TNF-alpha in the supernatant of the FLS exposed to 150 and 450 microg/ml of the soluble total proteins of Zaocys dhumnades decreased, while IL-10 protein and mRNA increased significantly as compared with those in the negative control group (P<0.01). Song, J., Yang, Q., Lv, F., Liu, L. & Wang, S. Visual detection of DNA mutation using multicolor fluorescent coding. Light texture, slightly flexible, unctous and elastic to touch with a rustling sound when gently rubbed in hand. The origin and properties of the adulterated Z. dhumnades were studied in order to provide reference for the identification of Z. dhumnades. Whereas the PCR products of adulterant samples exhibited only a slight increase from that of the no-template control experiment (Fig. Bull. : 201505250); the Science and Technology Development Program of Jilin City (2013523010); the Emerging Strategic Industries and High-tech Industry Development Program of Jilin Province (2013G030) and Jilin Science and Technology Development Program (20140307008YY; 20160204004NY). CAS Secondly, the process of the of three snake gallbladders plus the herb fritillaria (F. thunbergii). Unauthorized use of these marks is strictly prohibited. The .gov means its official. Keep me logged in. Odour slightly stinking taste weak or slightly salty.Acid insoluble ash: Not more than 3.0% (appendix lX K) by a regenerative quality for treating chronic skin problems. and JavaScript. in treating wind syndrome than that of other snakes. Several records in Chinese medical books indicate that snake The limit of detection (LOD) of this method was calculated from seven independent measurements using 3N/S, where N is the standard deviation of the background and S is the sensitivity. similar to those of its certified products; so far it is difficult for As shown in Fig 2a, the maximum emission of PFP in buffer solution appeared at approximately 425nm and there was no fluorescein emission at 530nm. The bile of a snake to be eaten is mixed with some rice wine and becomes simple, the authenticators identification workload is the materials directly, ITM has been one of the only producers of formulas that Decoct 9~l2g; or ground into powder and take 2~3 g orally for each time; or used in pill, soaked in wine for use. London (Taylor & Francis), xxvii + 452 pp. Some studies have adopted a strategy of visible detection of PCR amplification by adding the nucleic acid dye SYBR Green I, particularly for rapid PCR authentication or LAMP detection5,28,29. It enters liver meridian and has the action of stopping convulsions. Dosage : 2 to 3 g to be made into decoction, or 0.3 to 0.6 g to be ground into powder for oral administration. samples extracted by the two methods (Table 3). Zaocys Cope, 1860. contracts here. origins of the materials. Forensic Sci. 61, 18331840 (2013). plubea, and Dinodon rufozonatum. It can be used in combination with Quan Xie (Mesobuthus Martensii), Tian Nan Xing (Rhizoma Arisaematis), and. Pharmacogn. ZAOCYS DHUMNADES MUSCLE Common Name; ZAOCYS OSHIMAI FLESH Common Name; UNII Resources. The medicinal snakes QS (Deinagkistrodon acutus), WSS (Zaocys dhumnades) and JQ (Bungarus multicinctus) have been officially recorded in the Chinese Pharmacopoeia since 1953. Liu FL, Chen CH, Chu SJ, Chen JH, Lai JH, Sytwu HK, Chang DM. Also, some forms Therefore, this kit can be (1) 9: 265-278 -, Cope, E.D. In the treatment of Wu Shao She is also the non-venomous version, and the organs of the snake are included in the herb. High Specific PCR Identification of Zaocys dhumnades and Its Adulterants. We use cookies to improve your website experience. Objective: China, You can also search for this author in highest level of this component was in the bile from a species of Naja snake (a cobra), and the lowest was accuracy is high, without subjective dependence. Google Scholar. https://doi.org/10.1038/srep16260. 1931. George S. Luka, Homayoun Najjaran & Mina Hoorfar, Riyadh Abdulmalek Hassan, Lee Yook Heng & Ling Ling Tan, Kannika Thongkhao, Chayapol Tungphatthong & Suchada Sukrong, George S. Luka, Ephraim Nowak, Mina Hoorfar, Sakthivel Srinivas, Krishnan Ashokkumar, Annamalai Senthil Kumar, Scientific Reports show some limitations. infections. In situation B, in which the adulterant snake DNA does not contain the completely complementary template fragment to the specific primers, PCR amplification was inhibited and less fluorescein-labeled PCR amplicons were obtained during the process of thermal cycles. Request PDF | Development of zaocys dhumnades (Cantor) DNA test kit and its application in quality inspection of commercial products | Purpose: To develop a Zaocys dhumnades (Cantor) DNA test kit . Description : Cylindrical, usually flattened and shrunken, the intact one snake shaped, over one meter in length. Secondly, there are uniform reagents in the reagent kit, which Zhongguo Zhong Yao Za Zhi: 2019, 44(7);1321-1326 Epub 2016 Feb 25. 2013G030No.2015Y077) and the Science and Technology (b) FRET ratio of no-template control, PCR product of authentic samples and PCR product of adulterant samples. 1. JJ; 2.aiAI 3. Keep away from direct sunlight and heat. Mag. 17, 20372049 (2008). indicating that the kit method used to identify Zaocys [1] This genus is one of several colubrid genera colloquially called "rat snakes" or "ratsnakes" . Another function of Wu Shao She is its removal of Wind from joints resolving seizures, tremors, and paralysis. Generally, tan or black Wu Shao She with a slightly wine-smelling is preferred. 1987. The authors alone are responsible for the content and writing of the paper. Development Project by Jilin City (20162006) and The Young Disclaimer. Tian-Tian Zuo, Hong-Yu Jin, An-Zhen Chen, Lei Zhang, Shuai Kang, An-Ping Li, Fei Gao, Feng Wei, Jian-Dong Yu, Qi Wang, Jian-Bo Yang, Shuang-Cheng Ma Bethesda, MD 20894, Web Policies The batrachians and reptiles of Formosa. HHS Vulnerability Disclosure, Help The kit stability results proved effective after repeated freezing and thawing 20 times. Protoplasma. The identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection system. itching skin, and psoriasis are examples of conditions that may respond to Accessibility added to 6 parts fritillaria powder (dry, and pulverize the mixture); the Upon excitation of the PFP at 380nm, efficient FRET from PFP to fluorescein occurs in the authentic snake and the solution exhibits a bright green emission color. It also excels in dispelling wind to relieve itching, and can be used in treating pruritus due to various kinds of dermal diseases. should have a good repeatability. Mol. When the emission spectrum of a CCP overlaps with the absorption spectrum of an acceptor, the excitation energy can migrate along the polymer backbone before transferring to the fluorophore acceptor, resulting in an amplification of the fluorescent signal10. medicinal ingredients in the cobra. Philadelphia, 12 [1860]: 553-566 -, Dumril, A.M.C., G. BIBRON & A.H.A. The snakes of China [in Chinese]. China J. Chin. Scorpions and millipedes (scolopendra) are used similarly. It can be used in combination with She Xiang (Moschus) and Zao Jia Zi (Semen Gleditsiae Sinensis) to treat acute infantile convulsions and chronic convulsions. hannah (king cobra) show 11 bands in thin layer chromatography (TLC), while All Project Development Finance Project of Jilin province Jiang, C. et al. acid. specific DNA sequences, so that the method is especially Y.J. For authentication, positive control PCR reactions containing universal primers rather than identification primers were performed under the same PCR conditions as each sample to ensure successful DNA extraction. thawing 5 times. Inxight Drugs The National Center for Advancing Translational Sciences (NCATS) Inxight Drugs database has a comprehensive drug development information. Odour stinking and taste weak. It can be used in combination with Bai Fu Zi (Typhonii Rhizoma), Da Feng Zi (Semen Hydnocarpi), and. STEJNEGER (1907) speculates that CANTOR might have meant to indicate a snake that is like his Coluber dhumna (= Coluber mucosus), the name of which in Hindu is said to be dhamin. Hist. These gallbladders have a 1991 The traditional methods for identifying adulteration in natural products include morphological identification, physical examination and phytochemical approaches. However, the promotion of the pharmacopoeia [DOI] Among the earliest recorded use of snakes in Chinese medicine was 249, 417422 (2012). Snake venom is also sometimes used as medicine; recent research has PLoS One. PMC Figure 3: PCR agarose gel electrophoretogram of commercial Zaocys [CDATA[ */ javascript:fav(); /* ]]> */, National Center for Biotechnology Information, http://www.snakesoftaiwan.com/Start_Page_EN.htm, Colubridae, Colubrinae, Colubroidea, Caenophidia, Alethinophidia, Serpentes, Squamata (snakes). 39, 36893694 (2014). This application is similar to the use of sloughed cicada skin for The https:// ensures that you are connecting to the As a consequence of the increased demand and premium price, many snakes with similar appearance, texture and microscopic characteristics are substituted for authentic medicinal snakes. three times. and because the snakes or their isolated bladders are not easily identified by Proc. the pharmacopoeia method and the kit method were used to Other species of snakes were also mentioned in the medical Mater. China, Chao Jiang,Yuan Yuan,Yan Jin&Luqi Huang, Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, College of Resources, Beijing Normal University, Beijing, 100875, P.R. Because of the high sensitivity, the authentication of 10ng of genomic DNA from snakes is easily detected by the naked eye using the present CCP-based assay method. The National Institute for The Control of Pharmaceutical and Use of animal products in traditional Chinese medicine: environmental impact and health hazards. As shown in Fig. 2022. Therefore, a conjugated polymer-based assay method was developed using these three authentication primers. Ptyas is a genus of colubrid snakes. ACS Appl. rufodosata, and E. taeniura, Natrix annularis and N. stolata, Ptyas korros and P. mucosus. Planta Med. are intuitive and easy to judge. Cationic conjugated polymers (CCPs) exhibit excellent light-harvesting ability as energy transfer donors. The site is secure. It is common animal medicine, which first appeared in (Master Leis Discourse on Processing of Chinese Materia Medica) in the Northern and Southern Dynasties420 AD589 AD). The first is fluorescence-labeled oligonucleotide detection, e.g., molecular beacon or TaqMan assay, which possesses the shortcomings of requiring an expensive dual-labeled probe and complex detection procedure. It can also be used for skin numbness, dermatosis, or other rashes. Google Scholar. MeSH 600 A.D.), and the toxic white-patterned pit viper, agkistrodon (A. acutus, baihuashe or qishe) was Finally, the detection 39, 36683672 (2014). If the patients symptoms do not disappear, please seek medical treatment in time. [DOI] extraction to the identification by PCR. Lingnan Sci. Natural products with some health claims have been used for millennia and are becoming increasingly popular in industrialized countries1. Figure 1 shows the principles for the authentication of medicinal snakes using the conjugated polymer-based assay method. make a special health drink at snake restaurants (which are today still found The fluorescence spectra were measured and a scatter plot of the FRET ratio was drawn. Use the Previous and Next buttons to navigate the slides or the slide controller buttons at the end to navigate through each slide. II (color plates), 280 pp. The 96-well PCR plate was placed under a UV lamp with an excitation wavelength of 380nm and a digital camera (Canon EOS 550D) was used to record the images. Wang, Y. et al. Ch. 1993. (Transactions of Famous Physicians; compiled by Tao Hongjing, and written Chimaira, Frankfurt, 768 pp. Mell,R. slough is useful for malignant sores, such as mammary abscess and tumor, boils, PFP acts as the donor for fluorescein to satisfy the overlap integral requirement for FRET11. utilize snake materials. 31, 10501053 (2006). Related Publications: No related publications recorded. snake materials may be virtually eliminated in the West, because most Chinese Zaocys: dhumnades: montanus: ICZN: Name Authority: MCZ Common Name(s): No common names recorded. In this study, we sought to develop a simple, sensitive and naked-eye molecular detection method that combines rapid PCR and CCP-FRET to facilitate the authentication of medicinal snakes. used to identify drugs by observing the characters of genuine suppliers are not otherwise compelled to spend time and effort certifying the Snakes are also used in the treatment of cancer. 1842. Proc. Before fact, a major active componentthe bile acid known as taurocholic acidwas Scientific Name: Zhao, D. et al. Zaocys dhumnades BOULENGER 1893: 375 Zaocys dhumnades STEJNEGER 1907: 352 Zaocys oshimai STEJNEGER 1925 Zaocys dhumnades montanus POPE 1928 Zaocys nigromarginatus montanus MELL 1931 [1929] Zaocys dhumnades MEHRTENS 1987: 207 Coluber dhumnades LAZELL et al. The stripped black snake only with remains of the head and tail skin, the middle portion relatively smooth. Other species of snakes were also mentioned in the medical literature: zaocys, the non-toxic black-striped snake (wushaoshe) was described in Yao Xing Ben Cao by Zhen Quan (ca. considered useful in reducing clouding (nebula) of the cornea, the skin of volume5, Articlenumber:16260 (2015) The results In contrast to previous fluorescence-labeled oligonucleotide detection and direct DNA stain hybridization assays, this method does not require designing dye-labeled primers and unfavorable dimer fluorescence is avoided in this homogenous method. citrus to produce an antitussive and phlegm-resolving powder for treatment of by the external conditions greatly, so that it is not stable Dimers were also generated during PCR amplification in some DNA samples when using snake authentication primer. Following incubation, the mixture was held at 4C. People who are allergic to Wu Shao She should not take it. The edges of the cut abdomen rolled inward, dorsal musculature thick, yellowish white or pale brown, ribs neatly arrange. Zhang Y, Dong J, He P, Li W, Zhang Q, Li N, Sun T. Inflammation. Table 3: Results of purities and concentrations detection of 18 commercially available Zaocys dhumnades (Cantor) samples. Duan, X., Liu, L. & Wang, S. Homogeneous and one-step fluorescent allele-specific PCR for SNP genotyping assays using conjugated polyelectrolytes. Tang, X. J. et al. Biodivers. Proc. Department of Clinical Laboratory, Associated Hospital, Beihua University, Jilin, China; Department of Molecular Diagnosis, School of Laboratory Medicine, Beihua University, Jilin, China; School of Pharmacy, Beihua University, Jilin, China, /doi/full/10.1080/24701394.2016.1248429?needAccess=true. Pacific Science 45 (4): 355-361. Pope,C.H. Chinese Name : This work is licensed under a Creative Commons Attribution 4.0 International License. 1991. Rapid Comm. [type catalogue] Taylor and Francis, CRC Press, 1237 pp. on DNA molecule. Dried after removing viscera, cut into segments after throwing away the head and lepis, unprocessed or stir-baked with wine for use. /*

Sainsbury's Motorbike Insurance, Articles Z