dextran hydrodynamic radius

Thermo Fisher Scientific. Table1 shows TEWL values measured immediately after the microporation of human and pig skins. 2008;28(5):64557. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. The increased TEWL values which were proportional to the number of microspores indicate SC disruption and formation of microchannels in the skin. Carbohydrate Research, 160, 205-214. 10K, and c M.W. This result could reflect the presence of some aggregated species within the lysozyme sample solution. A novel correlation for protein diffusion coefficients based on molecular weight on radius of gyration. (2008). Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. Pharm Res. The importance of this type of study lies in the analysis of the polysaccharide behavior at industrial processes, the requirements to reduce energy, avoid flow problems and product quality control. Biomaterials, 22, 1891-1898. Global hydrodynamic analysis of the molecular flexibility of galactomannans. Antoniou, E.; Buitrago, C. F.; Tsianou, M.; Alexandridis, P. (2010). 2012;101(8):28619. 2000;3(9):31826. Dextran Utilization During Its Synthesis by Weissella cibaria RBA12 Can Be Overcome by Fed-Batch Fermentation in a Bioreactor. The effect of branching is best recognized from the molecular weight dependencies of []. A simple and rapid method for evaluation of MarkHouwinkSaturada constants of linear random coil polysaccharides using molecular weight and intrinsic viscosity determined by high performance size exclusin chromatography: application to guar galactomannan. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. The human and pig skin permeability of FITC-dextran (M.W. Journal of Polymer Science, 17, 527-546. 2015;32(5):170413. Recent developments in polysaccharide-based materials used as adsorbents in wastewater treatment. dextran Journal of Applied Polymer Science, 27, 795-810. 2008;26(11):12618. Seven dextrans, provided by Sigma, with molecular weight of 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa. WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. The hydrodynamic radius is important in predicting transretinal penetration Carroll PJ. What is dextran? Application areas - TdB Labs AB Increased TEWL values are evidence of micropores formation. Figure 2 shows the classical method for calculating Mark-Houwink parameters, where a linear relation between the intrinsic viscosity and the molecular weight is established for each temperature. Carbohydrate Polymers, 39, 377-380. Bookshelf With FITC-dextran 20K, cumulative permeation in the human skin was 2.530.48M, and 3.340.19M in the pig skin, which significantly lower than FITC-dextran 4K. Cumulative amount of FITC-dextran permeated after microporation on the human and pig skin for FITC-dextran M.W.. a M.W. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC dextran measured in plasma 1 h after its oral application is a marker of small intestinal permeability. Effect of temperature on the intrinsic viscosity and conformation of chitosan in dilute HCl solution. Peptide and protein transdermal drug delivery. These constants can be determined experimentally by measuring the intrinsic viscosity of several polymer samples for which the molecular weight has been determined by an independent method (i.e. The a value is higher than calculated in this work, because these calculations were performed with number average molecular weight (Mn) which rearranges the value of a and forces him to enter into unbranched or linear molecules (a > 0.5), on the other hand, if molecular weights (Mw) ware used to value would be less than 0.5. These results indicate that healing and regeneration occurred in the human cadaver skin after the microporation. 4K, b M.W. Mitragotri S. Mechanical disruption of skin barrier for vaccine delivery. 2012;27(3):20212. Carbohydrate Polymers 87, 405-410. In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). (1990). Regulating dynamic signaling between hematopoietic stem cells The values of Mark-Houwink parameters could be universalized with certain precautions, being an indication for the calculation of molecular weight in the temperature range of 20-40C. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. Int J Pharm Investig. Weissella: An Emerging Bacterium with Promising Health Benefits. An undergraduate physical chemistry experiment. 2023 BioMed Central Ltd unless otherwise stated. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. Viet, D.; Beck-Candanedo, S.; Gray D. G. (2008). Smith, David R.; Raymonda, John W. (1972). The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. Ind. The Mark-Houwink a constant is close to 0.5 in poor solvents. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. (2008). 2011;5(2):95132. Carbohydrate Research, 340, 811-826. J. Braz. Expert Opin Drug Deliv. dextran Dextran 670 GPC Standard | Pharmacosmos Dextran Kasaai, Mohammad R. (2012). 2014 Sep;5(9):2324-30. doi: 10.1039/c4fo00319e. Human cadaver skin has been confirmed as alive skin tissue through analysis of its morphology and enzymes, and its usefulness as an alternative skin membrane in drug permeation experiments has been verified [20]. Armstrong, J. K.; Wenby, R. B.; Meiselman, H. J.; Fisher, T. C. (2004). To overcome these limitations, microporation devices that act as therapeutic vehicles for delivering drug molecules across the skin barriers have been developed. 2003;89(2):31120. FOIA Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. a Immediately after microporation, b 4h, c 8h, and d 24h (n=5, *: p<0.05). Masuelli, Martin Alberto (2013). Ioan, C.E. Keywords: Sci. Pamis, R.; Hernndez Cifre, J. G.; Lpez Martnez, M.C. De Belder, A.N. After 24h, the cumulative permeation of FITC-dextran M.W. Journal of Applied Polymer Science, 13, 2031-2032. a-d Haematoxylin/eosin-stained histological sections of human cadaver skin sample and e quantified data using ImageJ for different time periods after microporation. Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC Correspondence to Determination of the intrinsic viscosity has been the most used measurement in the last 60 years. Part of The exponent a is a function of polymer geometry, and varies from 0.5 to 2 and exponent is dimensionless. Monolayers and Thin Films of Dextran Hydrophobically Modified. Biomacromolecules 8, 2464-2475. Journal of Applied Polymer Science, 72, 871-876. WebIn this regard, we chose as crowding agents a number of polymers with hydrodynamic dimensions comparable to those of iRFP713 in different structural states: polyethylene glycol8,000(PEG-8000)withaneffectivehydrodynamicradiusof24.5 1.9(Devanand & Selser, 1991), Dextran-40 and Dextran-70 with hydrodynamic radius of 44.550 and 5864 , Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. This study demonstrated that hydrodynamic volume and radius of gyration of a dextran sample with M w < 20 kDa and its linear counterpart with equal M w are almost Results and Discussion The microscopic dynamics of proteins are probed in a light scattering experiment. Before Critical parameters for gel permeation A similar article of dextran/water [35] clearly demonstrating the dependence of Mark-Houwink parameters with temperature. The microporation device based on radiofrequency can be used to enhance delivery efficiency by removing the epidermis layer. However, the currently available transdermal systems are limited by low drug permeability across the skin due to the lipophilic barrier function of the stratum corneum (SC), the outermost skin layer of the skin [3,4,5]; the methods favor low molecular mass, and lipophilic molecules, making it difficult to exploit the transdermal route for large and water-soluble molecules such as proteins and peptides. Careers. Prausnitz MR, Langer R. Transdermal drug delivery. J Cosmet Laser Ther. Figure 3 shows that the parameters vary with temperature, evidencing a clear functionality of them. US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES Levin G, Gershonowitz A, Sacks H, Stern M, Sherman A, Rudaev S, Zivin I, Phillip M. Transdermal delivery of human growth hormone through RF-microchannels. dextran Dextran Specifications: Label (Ex/Em): Fluorescein (494/521) Size: 40,000 MW. WebTry out PMC Labs and tell us what you think. For a sphere and a random coil 0 the values are 9.23x1023 mol-1 and 2.60x 1023mol-1, respectively. Chem. 24h after microporation, the pore sizes reduced from 64.8817.20m (0h) to 48.854.27m (24h), and the pore depths reduced from 83.9120.56m (0h) to 63.9527.61m (24h). (1954). Article Soc., 21, 1, 78-86. Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. Figure3 shows a magnified image of human cadaver skin with microchannels formed by the microporation device (250kHz) and the skin closure over time. In our present study, microporated skin permeated insufficient amount of 20K FITC-dextran, suggesting that enhancement of permeability was unsuccessful with macromolecules of more than 20KM.W.. Carbohydrate Polymers 72, 356-360. Cecopieri-Gmez, Martha L.; Palacios-Alquisira, Joaqun (2005). WebAmines per 70KDa dextran Theeffectofchargerepulsiononthe hydrodynamicradiuscanbeseenwithRh increaseswithchargedensity.Charge repulsioncausesthehydrodynamicradius toincrease.Withincreasingcharge,the dextranprogressivelyunfolded.Evena lowlevelofaminationcausesthepolymer tounfold. Chem. These hydrodynamic parameters show that these polysaccharides behave as a compact rigid sphere and contract by the increase of temperature (RH decreases) for the Mw range from 8.8 to 200kDa. Eremeeva, T. E.; Bykova, T. O. ; Ross-Murphy, S.B. 56 The linkage that the enzyme dextranase breaks down is indicated by the black arrow. Sung-Wook Choi. Biophysical Journal, 87, 4259-4270. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). Carbohydrate Polymers, 68, 477-488. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). Synthesis of dextran by dextransucrase treated, sucrose-supplemented skimmed milk revealed the promising potential of dextran as a food additive. Transdermal drug delivery system for non-steroidal anti inflammatory drugs: a review. HSE and DK helped to porate the skin and to measure TEWL. Polysaccharide hydrogels for modified release formulations. Search principles of dynamic light scattering These modifications include degradation of hemidesmosomes attached to the dermis, degradation of desmosomes linked to adjacent cells, contraction of intracellular tonofilament, and lamellipodia formation. Masuelli, Martin Alberto. All possible degrees of curling can be displayed by any molecule, but there will be an average configuration which will depend on the solvent. Masuelli, Martin A. Hydrodynamic properties of whole arabic gum. 2012;87(6):1728. 4h after microporation, the sizes of the micropore decreased slightly. ; Wood, P.J. (1969). Epub 2012 Oct 18. Enhanced topical delivery of small hydrophilic or lipophilic active agents and epidermal growth factor by fractional radiofrequency microporation. Viscosity of in water solution polysaccharides depends on intrinsic characteristics of the biopolymer (such as molecular weight, volume, size, shape, surface charge, deformation facility, esterification degree, and galacturonic content) and on ambient factors (such as pH, temperature, ionic strength, solvent, etc.). (2004). Food Funct. For data requests, please contact the authors. After 8h, the micropore became more distorted from the previous circle, suggesting that the micropores were closing. A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. Pharm Res. In this sense, the largest deviations were obtained for the highest molecular weight (580-2000kDa with values from 0.8467 to 0.7346). 2019;9(3-S):9726. Skin barrier disruption by acetone: observations in a hairless mouse skin model. The genus Weissella: taxonomy, ecology and biotechnological potential. For a limited temperature range, the viscosity of a polymer solution varies generally following a relation similar to that of usual liquids (rrhenius plot), where the polymer concentration is not very high and that temperature remains far enough from glass transition [38], and for system PEAorganic solvents see reference [63]. Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was The flattening of the curves at higher molecular weight is in agreement with an increased branching density. Drug Deliv. Colloid and Polymer Science, 286, 1223-1231. It is a measure of the width of molecular weight distributions (MWD) as well as probability of molecular weight distribution curve (GPC). The Hydrodynamic Radii of Macromolecules and Their Effect on Red Blood Cell Aggregation. Clipboard, Search History, and several other advanced features are temporarily unavailable. Porcine wound models for skin substitution and burn treatment. Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. Unperturbed dimensions and theta temperature of dextran in aqueous solutions. Macromolecules. California Privacy Statement, Bahary, W. S.; Hogan, M. P.; Jilani, M.; Aronson, M. P. (1995). These values indicate for dextrans in dilute aqueous solution, both methods give very similar results of the intrinsic viscosity and the respective Mw. The above data are not useful because it can not be compared with those obtained in this work. Anal. Chem. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. Saudi Pharm J. After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining. Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. Google Scholar. Thus, typically, the dextran glomerular for a molecule with a Stokes-Einstein (SE) radius of 30 is approximately sevenfold higher than that of neutral horseradish Eng. Intrinsic viscosity molecular weight relationship. 2011;16(2):518. Alternatives to animal testing: a review. For 40 kDa molecules, the hydrodynamic diameter dh ~8 nm is much smaller than the collagen mesh size [ 33, 41 ]. ; Rjumtsev, E.I. The values of 0 decreases from 2.5429 to 2.3326 x 1023mol-1 demonstrating a relative flexibility of particles. Carbohydrate Polymers, 36, 319-326. Microporation techniques for enhanced delivery of therapeutic agents. Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. Thomas Gutberlet (Lipoteichoic Acid): Statistiques d'influence The formula is verified for different systems of polymer-solvent and the values are in accord with those obtained by extrapolation. WebThe Stokes radius or StokesEinstein radius of a solute is the radius of a hard sphere that diffuses at the same rate as that solute. dextran hydrodynamic radius WebFluorescein isothiocyanatedextran average mol wt 60,000-76,000; CAS Number: 60842-46-8; Synonyms: FITCDextran; find Sigma-Aldrich-FD70S MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich Microporations were performed 2, 5, and 10 times and the skin damage was confirmed using the TEWL measurement. Unauthorized use of these marks is strictly prohibited. Formation of dextran hydrogels by crystallization. With FITC-dextran 20 K, cumulative Microneedles and their applications. (2013). In vivo iontophoretic delivery of salmon calcitonin across microporated skin. Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1), These structural differences can significantly affect FITC-dextran delivery due to even after microporation [30]. In a good solvent, a temperature increase results in an intrinsic viscosity decrease and in a less-extended conformation (RH<), because the entropy value increases with an increase in temperature and it is unfavorable for an extended conformation. Journal of Chemical Education, 80(9), 1036-1038. Format), Citation-(EndNote Viscotek GmbH, Europe. Harding, Stephen E. (2005). Influence of molecular weight on Fractional thermolysis by bipolar radiofrequency facilitates cutaneous delivery of peptide and siRNA with minor loss of barrier function. Mainaa, Ndegwa Henry; Pitknen, Leena; Heikkinen, Sami; Tuomainen; Pivi; Virkki, Liisa; Tenkanen, Maija (2014). The micropore formed at the SC through to the epidermis, enabling drug delivery. Universal size-independent quantities for the conformational characterization of rigid and flexible macromolecules. Dextrans can function efficiently as anterograde or retrograde tracers, European Journal of Pharmaceutics and Biopharmaceutics, 68, 319-329. WebR H Hydrodynamic radius [m] The basic DLS setup. In the case of the work performed in references [34, 35, 36] they studied using the number average molecular weight, which rearranges the value of a and forces it to enter into unbranched or linear molecules, on the other hand, if molecular weights are used the value would be less than 0.5. Dextran Fractions. In vitro studies with human cadaver skin is a viable, ethical, and economical alternative for in vivo animal studies. Gekko, K. (1971). Intrinsic viscosity determination offers a simple and useful method that requires low cost equipment and yields useful information on soluble macromolecules. In addition, the permeation rate was higher in pig skin than in human skin. 1994, 95-0355. For a rigid or rod like polymer molecule that is greatly extended in solution, the Mark-Houwink a constant approaches to a value of 2.0 [48].The hydrodynamic radius (RH), for a sphere ((a/b) = 2.5) is given by the Einstein relation [49]. 10% neutral buffered formalin was purchased from Hisko (Gunpo, Korea). In summary, we demonstrated effective enhancement of skin permeability and delivery of macromolecules lower than 20KM.W., and regeneration of human cadaver skin after microporation with an RF-based microporation device. What is hydrodynamic radius WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol Figure5 shows the cumulative amount of the FITC-dextran (M.W. Drug deliv. Pavlov, G.M. and transmitted securely. At 20C data "a" value obtained in this work is intermediate to those given by references [1, 37] while the value of kM-H is lower. FITC-dextran solution was prepared at 1mM in PBS. Nanoparticles in magnetic resonance imaging: from simple to dual US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES Kloster, C.; Bica, C.; Lartigue, C.; Rochas, C.; Samios, D.; Geissler E. (1998). Accueil; Solution; Tarif; PRO; Mon compte; France; Accueil; Solution; Tarif; PRO; Mon compte Granath, K. A. Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. The skins were from the abdomen of a 39-year-old caucasian woman (BMI: 24). Characterization and Classification of Dextrans from Ninety-six Strains of Bacteria. The first term on the left-hand side of equation 2 (i/c) has a linear relation as a function of the concentration (c in g/cm3), where is the intrinsic viscosity [] is given by (c0) [42, 43]. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Microporation was performed 2, 5, and 10 times on each sample. The unfolding of iRFP713 in a crowded milieu - PeerJ Equivalent Radii and Ratios of Radii from Solution Properties as Indicators of Macromolecular Conformation, Shape, and Flexibility.

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